Near Infrared Fluorescence Imaging

Fluorescence imaging has become a fundamental tool for biomedical applications; nevertheless, its intravital imaging capacity in the conventional wavelength range (400–950 nm) has been restricted by its extremely limited tissue penetration. To tackle this challenge, a novel imaging approach using the fluorescence in the second near-infrared window (NIR-II, 1000–1700 nm) has been developed.

Near infrared fluorescence imaging. Near-infrared fluorescence (NIRF) imaging is an emerging clinical technology that requires administration of a fluorescence-imaging agent that can be excited at near-infrared (NIR) wavelengths of ≥760 nm. Upon illuminating tissue surfaces with penetrating NIR light to excite the imaging agent within the tissues, the generated fluorescence is. A nitroreductase and glutathione responsive nanoplatform for integration of gene delivery and near-infrared fluorescence imaging† Hong Liang , ‡ a Qunjie Bi , ‡ a Ao Hu , a Xiaobing Chen , a Rongrong Jin , a Xu Song ,* ab Bowen Ke , a Matthias Barz c and Yu Nie * a Objectives This study sought to determine whether indocyanine green (ICG)–enhanced near-infrared fluorescence (NIRF) imaging can illuminate high-risk histologic plaque features of human carotid atherosclerosis, and in coronary atheroma of living swine, using intravascular NIRF-optical coherence tomography (OCT) imaging. Background New translatable imaging approaches are needed to identify. In 2017, Bracco Imaging entered into the near-infrared imaging market with the acquisition of SurgVision.Surgvision is a start-up company focused on developing fluorescence image-guided surgery platform solutions using high-definition cameras and tracers.

Fluorescence bioimaging: Near-infrared fluorescence imaging of living subjects Date: June 4, 2020 Source: Wiley Summary: Scientists can monitor biomolecular processes in live tissue by noninvasive. Near-Infrared Fluorescence Imaging System This page introduces a surgery support system for medical professionals and includes clinical images during surgery. Near-Infrared Fluorescence Imaging System - LIGHTVISION NEW. High Definition Intraoperative ICG Fluorescence Imaging System. First experience on laparoscopic near-infrared fluorescence imaging of hepatic uveal melanoma metastases using indocyanine green, 2014 4 Boni et al., ICG-Enhanced Fluorescence-Guided Laparoscopic Surgery, Doctor-to-Doctor Manual ENDO-PRESS®, (ISBN 978-3-89756-934-8) To help prevent bile duct injury, we have previously described the use of intraoperative near-infrared (NIR) fluorescence imaging to visualize the extrahepatic bile ducts in real time (8, 9). Due to possible anatomical variations of the hepatic artery, unexpected laceration and hemorrhage is also a major complication of LC (10–13).

While it is not meant to serve as an exhaustive summary of ICG use in surgery, the goal is to highlight the successful use of this technology in a number of settings. As the technology and applications continue to expand, Video Atlas of Intraoperative Applications of Near Infrared Fluorescence Imaging serves as a foundation upon which to build. Herein, a macromolecular reporter (CyP1) was synthesized for real‐time near‐infrared fluorescence (NIRF) imaging and urinalysis of BC in living mice. Because of the high renal clearance (ca. 94 % of the injection dosage at 24 h post‐injection) and its cancer biomarker (APN=aminopeptidase N) specificity, CyP1 can be efficiently transported. Near-infrared (NIR) fluorescence imaging has improved imaging depth relative to conventional fluorescence imaging in the visible region, demonstrating great potential in both fundamental biomedical research and clinical practice. To improve the detection specificity, NIR fluorescence imaging probes have been 2020 Chemical Science HOT Article Collection Lactosome is a core-shell-type polymeric micelle, and enclosing labeling or anticancer agents into this micelle enables drug delivery. In this study, we investigated the diagnostic and therapeutic efficacies of indocyanine green (ICG)-loaded lactosome for near-infrared fluorescence (NIF) imaging and photodynamic therapy (PDT) for HCC.

Moreover, photosensitizer, an essential element of PDT, can also be employed in cancer imaging diagnosis as it can emit the near-infrared (NIR) light , . Therefore, the NIR photosensitizer like chlorin e6 (Ce6) is widely used for the research of both cancer PDT treatment and fluorescence imaging [8] , [11] , [12] , [13] . This review focuses on recently published examples of in vivo near-infrared (NIR) fluorescence imaging. To be successful, each example addressed a common set of parameters (outlined below and summarized in Box 1) and optimized the signal to background ratio (SBR; also called signal to noise ratio). Local recurrence is the main cause of death among patients with oral squamous cell carcinoma (OSCC). This study assessed near-infrared fluorescence (NIF) imaging and spectroscopy to monitor surgical margins intraoperatively for OSCC. Cytological and animal experiments were first performed to confirm the feasibility of monitoring surgical margins with NIF imaging and spectroscopy. We present confocal fluorescence lifetime imaging microscopy in the second near-infrared (NIR-II) window to assess the morphological and biochemical information of live samples. A home-built superconducting single-photon detector (SSPD) was used to facilitate the NIR-II fluorescence lifetime measurement. The SSPD has many advantages, including high sensitivity to NIR-II signals (detection.

The near‐infrared window of fluorescent heptamethine cyanine dyes greatly facilitates biological imaging because there is deep penetration of the light and negligible background fluorescence. But dye instability, aggregation, and poor pharmacokinetics are current drawbacks that limit performance and the scope of possible applications. Fluorescence bioimaging often uses the near-infrared light region because this radiation can efficiently penetrate human tissue.Fluorescent dyes designed for this purpose usually have a flat. The past decade has witnessed rapid technological development on nanoscale probes and imaging optics in the second near-infrared transparency window (NIR-II, 1000–1700 nm). These methods hold great promise for biomedical applications due to their deep penetration through tissues and high fidelity of images. However, applications of these techniques in biomedical research and translational. Near-infrared fluorescence imaging has the potential to improve the detection of malignant tissue during surgery, significantly improving outcome. Here, we report the use of OTL38, a near-infrared (796 nm) fluorescent agent, that binds folate receptor alpha, which is expressed in >90% of epithelial ovarian cancers.

Smartphone developer for multi-spectral, UV-VISIBLE-NIR, and fluorescence imaging applications supporting Google's Android and Apple's iOS platforms. We're specialized in modifying smartphone camera modules for near infrared imaging applications by removing the original near infrared cut-off filter.