Rna Oligo Synthesis

DNA oligos produced by GenScript's state-of-the-art oligo-synthesizer have substantial purity with low truncated sequences. Each oligo is meticulously monitored during synthesis and controlled according to GenScript's stringent quality assurance and quality control standards.

Rna oligo synthesis. After oligonucleotide synthesis and deprotection with ammonia, the 2′-TBS groups are removed from the oligoribonucleotide using a source of fluoride ion (e.g. tetrabutylammonium fluoride or Et 3 N · 3 HF). Removal of TBS protecting groups proceeds smoothly without cleavage or isomerization of the phosphodiester linkage. Oligonucleotides produced with the Dr. Oligo line of dna synthesizers can be used for Oligonucleotide and Gene synthesis, PCR / RT-PCR , siRNA / RNAi , miRNA, CRISPR Cas9, DNA / RNA sequencing , Immunity and Biochemistry , High-Throughput screening , Natural Product Biosynthesis , Gene Construction , Reverse Transcription PCP , Hybridization , SNPS , other diagnostic or therapeutic research. Bio-Synthesis supplies Custom RNA Oligo Synthesis services for a wide range of labels, modifications, scales and purities. We provide RNA synthesis for long RNA oligos up to 300 bases. RNA oligos are synthesized using the latest nucleic acid technology and are purified using RNase free PAGE electrophoresis combined with HPLC. The random sequence enables the unbiased binding of primers to anywhere on most types of RNA including rRNA and small RNAs. Since they bind throughout, the majority of the gene products should be covered, therefore increasing the chance of amplification during PCR. Oligo(dT) primers. Oligo(dT) primers are composed of stretches of deoxythymidine.

Synthesis scale: 10 nmol • 40 nmol • 200 nmol • 1000 nmol • 2.5 μmol • 5 μmol • 10 μmol • 20 μmol 1; Backbone: DNA, RNA, LNA®, 2’O-Me RNA, 2’-O-MOE, PNA 2, phosphorothioate, methylphosphonate and all linkages; Modification: More than 300 modifications! Purification: Synthetic RNA is widely used in research applications as an important tool for gene functional analysis and the development of novel therapeutic strategies. GenScript provides high-quality and cost-effective custom RNA oligos with flexible synthesis scales to meet researchers' different needs. IDT’s long-standing reputation as a pioneer and leader in custom oligo manufacturing is primarily due to our proprietary synthesis platforms. Most components in our manufacturing process are designed and developed in-house, including specialized synthesizers that accommodate the most demanding oligo requests and high-throughput automation. We at Bio Basic have modified our oligo synthesis process to allow a simpler and more consistent approach to this pricing structure and final yield deliverable. Because of this we guarantee the total yield of each oligonucleotide as a FIXED final yield in OD (Optical Density) units.

*Minimum Yield based on oligos in tubes from 20–45 bases, inclusive. Note: PAGE purification is available for oligos greater than 20 bases in length. 2-Step purification is required for HPLC-purified oligos greater than 60 bases in length, and for PAGE-purified oligos greater than 69 bases in length. Oligonucleotides produced with the Dr. Oligo line of dna synthesizers can be used for Oligonucleotide and Gene synthesis, PCR / RT-PCR , siRNA / RNAi , miRNA, CRISPR Cas9, DNA / RNA sequencing , Immunity and Biochemistry , High-Throughput screening , Natural Product Biosynthesis , Gene Construction , Reverse Transcription PCP , Hybridization , SNPS , other diagnostic or therapeutic research. RNA oligos are short, single- or double-stranded synthetic RNA sequences that can be used in nearly any RNA-specific molecular biology application. Using the Oligo Entry ordering tool, you can design your sequences to contain unmodified RNA bases, 2′-O-methyl RNA bases, or chimeric DNA bases. In many applications, oligo(dT) or random primers can be used to prime cDNA synthesis. Oligo(dT) primers are used to amplify mRNA. However, use of oligo(dT) results in referential priming at the 3' end of the RNA.

Single-stranded RNA oligos up to 120 bases, desalted and delivered dry in tubes. Ultramer RNA Oligonucleotides are manufactured using proprietary synthesis methods that deliver high quality, long RNA oligos. Each oligo is quality controlled by ESI mass spectrometry*. Turnaround time is 6–8 business days. Mid and Large Scale Oligo Synthesis Learn More. TriLink specializes in mid-scale and large-scale synthesis (50 mg to multiple grams) of both DNA and RNA oligonucleotides. Mid and Large Scale Oligo Synthesis DNA & RNA oligo synthesis We provide custom designed dual labeled probe set, including one probe and a pair of primers. Regular package: one vial of probe (5 OD) with 5’ FAM (reporter) and 3’ TAMRA (quencher), and two vials of primers (2 OD each). Custom RNA oligos » Single-stranded and duplexed RNA sequences, manufactured to your specifications. Import multiple sequences from an Excel or text file and select from hundreds of modifications. Choose from a variety of custom services to meet your research needs.

DNA & RNA; Custom DNA oligos; Custom RNA oligos; Affinity Plus DNA & RNA oligos; DNA oligo pools; Large-scale synthesis; SameDay oligos; Inventoried oligos; Oligo modifications; Genes & gene fragments; Double-stranded DNA fragments; Single-stranded DNA fragments; Custom gene synthesis; DNA Origami; Next generation sequencing; Adapters; Library. Synthesis; Purification; Quality control; Chemical Modification; Dissolving and storage; Calculations;. - unmodified RNA up to 120 bases - RNA oligo annealing - XL and XXL RNA (5-50 mg) - RNA modifications - 2´-modified RNA. - smiFISH Oligo Pools. GENEWIZ provides a suite of oligo synthesis options to meet the ever-changing needs of the molecular bench scientist. From oligos for Sanger sequencing and PCR to oligos modified with your choice of over 200 chemical modifications, our oligo synthesis services provide the high quality results you expect from GENEWIZ with the breadth of oligo synthesis expertise you need for your experiments. From applications like gene synthesis, genetic diagnostic test kits, PCR/RT-PCR, siRNA/RNAi, miRNA, CRISPR Cas9 and DNA/RNA sequencing – to broader areas like evolutionary history, forensics, immunotherapy, diagnostic and therapeutic research – to high-throughput screening, modified oligos, mixed backbones, LNAs and the visual detection of.

Custom RNA synthesis by Dharmacon™ offers experimental flexibility with limitless modifications, greater yields and faster delivery than any other synthesis service. Preclinical oligo synthesis Get oligonucleotides to the clinic faster! Expedite the journey with a reliable, experienced supplier of research, preclinical, and OEM.